In vivo and in vitro effects of Rhizopus extract on body growth and steroid production in masu salmon, Oncorhynchus masou Brevoort

Abstract

This study investigated the in vivo and in vitro effects of Rhizopus (filamentous fungus) extract (RU) in masu salmon Oncorhynchus masou Brevoort. Underyearling fish were fed with RU for 16 months. Monthly changes in body growth, gonadal maturation and serum levels of sex steroids were monitored. Gonads were also incubated at 0, 1, 10, 100 and 1000 μg RU mL−1 Leibovitz's L-15 medium for 18 h. The levels of steroids in serum and cultured medium were measured. It was determined that RU-fed immature and mature males, when compared with control groups, showed significantly higher body growth during spring, summer and the spawning period. Similarly, immature RU-fed females showed significantly higher fork length and body weight in autumn, spring and summer. Furthermore, RU-fed males showed significantly higher levels of serum testosterone (T) and 11-ketotestosterone (11-KT) levels in the pre-spawning season, and 17α, 20β-dihydroxy-4-pregnen-3-one (DHP) in the spawning season. In vitro RU incubation of gonads showed a dose-dependent and significant increase in T, 11-KT, oestradiol-17β and DHP release in the medium. It appears that the causes of enhanced body growth and increased steroid production herein observed in salmonids are the physiologically active substances that may be present in the mycelium of the fungus.