In vivo and in vitro effects of homocysteine on Na+,K+-ATPase activity in parietal, prefrontal and cingulate cortex of young rats

Abstract

In the present study we determined the effect of chronic administration of homocysteine on Na+,K+-ATPase activity in synaptic membranes from parietal, prefrontal and cingulate cortex of young rats. We also studied the in vitro effect of homocysteine on this enzyme activity and on some oxidative stress parameters, namely thiobarbituric acid-reactive substances (TBA-RS) and total radical-trapping antioxidant potential (TRAP) in the same cerebral structures. For the in vivo studies, we induced elevated levels of homocysteine in blood (500μM), comparable to those of human homocystinuria, and in brain (60nmol/g wet tissue) of young rats by injecting subcutaneously homocysteine (0.3–0.6μmol/g of body weight) twice a day at 8h intervals from the 6th to the 28th postpartum day. Controls received saline in the same volumes. Rats were killed 12h after the last injection. Chronic administration of homocysteine significantly decreased (50%) Na+,K+-ATPase activity in parietal, increased (36%) in prefrontal and did not alter in cingulate cortex of young rats. In vitro homocysteine decreased Na+,K+-ATPase activity and TRAP and increased TBA-RS in all cerebral structures studied. It is proposed that the alteration of Na+,K+-ATPase and induction of oxidative stress by homocysteine in cerebral cortex may be one of the mechanisms related to the neuronal dysfunction observed in human homocystinuria.