Abstract
Analysis of enzyme activities and metabolites in the rhizosphere requires suitable growth systems. Here, we describe the use of plastic syringes as a test system for measuring extracellular root enzyme activities of a single seedling under sterile conditions. Syringes have been originally used to analyze hydrolysis of rhizobial nodulation signals (Nod factors) by host legumes such as Medicago truncatula. The developed method offers now the opportunity to use syringes for other purposes. Syringe systems can be used to determine any enzyme activity in the rhizosphere, providing that the substrates and products in the growth medium are water-soluble, nontoxic, and detectable. Moreover, syringes can be employed to collect root exudates from a single seedling.
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