Abstract
This study reports a protocol for the germination and early seedling growth of Pinus peuce Gris. using zygotic embryo culture. In order to overcome seed dormancy and optimize organogenesis, the effect of nutritional, plant growth regulatory and physical factors on in vitro germination and growth of isolated mature zygotic embryos of P. peuce were investigated.
Highlights
Pinus peuce Gris. (Macedonian pine) is a Tertiary relic species endemic to the Balkan Peninsula
Non-stratified seeds have an extremely low germination rate, which is partly due to the hard seed coat that acts as a physical barrier, and due to low and extremely variable seed viability (Nikolić, 2005)
The method of substituting the megagametophyte with nutrients, plant growth regulators and physical factors is increasingly applied for successful breaking of seed dormancy, shortening the life cycle and allowing the rapid multiplication and conservation of endangered conifer species or species with extremely low germination rate
Summary
Pinus peuce Gris. (Macedonian pine) is a Tertiary relic species endemic to the Balkan Peninsula. (Macedonian pine) is a Tertiary relic species endemic to the Balkan Peninsula. Because of the limited area of P. peuce natural distribution, variable abundance of seed production and low ability for natural reproduction, the implementation of conservation measures for this economically important forest plantation species is required. Zygotic embryo culture has been shown to play an important role in rapid in vitro propagation of endangered forest tree species to overcome physical and biotic interference (Rambabu et al, 2006). The method of substituting the megagametophyte with nutrients, plant growth regulators and physical factors is increasingly applied for successful breaking of seed dormancy, shortening the life cycle and allowing the rapid multiplication and conservation of endangered conifer species or species with extremely low germination rate. Ducted to evaluate the requirements for in vitro germination and growth of Macedonian pine zygotic embryos, in order to optimize organogenesis using mature zygotic embryos as the starting explants
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