Abstract
Two promoters, Pl and P2, have been identified upstream of the structural gene for the 16S rRNA in spinach chloroplasts (Lescure et al. 1985). A highly purified soluble spinach chloroplast RNA polymerase is shown to initiate correctly in vitro the transcription of the ribosomal DNA operon of spinach chloroplasts at the PI promoter. The P2 promoter is used only by the E. coli RNA polymerase either in vivo or in vitro. However, the P1 promoter is used at a higher frequency than the P2 promoter by the heterologous system. Both promoters appear to be used more efficiently if the DNA template is in a supercoiled form. The P2 promoter is not used in vivo in chloroplasts even if the processing of RNAs is blocked by using lincomycin.
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