In vitro study of biocompatibility of a wrapped-core structure composite scaffold for bone tissue engineering

Abstract

Objective To assess whether a wrapped-core structure composite scaffold possesses the biocompatibility in vitro.Methods The wrapped-core structure composite scaffold composed of poly lactic-co-glycolic acid (PLGA)/β-TCP skeleton wrapped with type Ⅰ collagen was manufactured via LDM,and PLGA/β-TCP scaffold was chosen as the control group.SEM was used to study morphology of bone marrow mesenchymal stem cells (BMSCs) on the surface of the original scaffolds.Hydrophilicity was estimated by water absorption ratio.Cell proliferation was estimated by methyl thiazol tetrazolium (MTT) assay and the osteogenic differentiation of BMSCs was estimated by alkaline phosphatase (ALP).Results The water absorption of the original scaffold [(18.1 ± 0.4) ％] was significantly higher (P ＜ 0.01) than that of the control group [(2.6 ±0.3)％].After co-culture of BMSCs for 6,10,and 14 days,the A values of original scaffolds and control groups were 0.28 ± 0.09,0.60 ± 0.10,0.76 ± 0.10,and 0.19 ± 0.09,0.36 ±0.05,0.46 ± 0.12,respectively; the activities of ALP were 124.33 ± 14.22,189.00 ± 19.52,230.00 ± 12.12,and 78.33 ± 8.02,105.67 ± 7.77,123.67 ± 20.84,respectively.There was significant difference between the two groups (P ＜ 0.05).Conclusion The wrapped-core structure composite scaffold possesses admirable biocompatibility and is expected to be of useful value in bone tissue engineering. Key words: Bone tissue engineering; Scaffold; Biocompatibility