Abstract
An efficient protocol for plant regeneration of Carica papaya through indirect somatic embryogenesis was developed. Initially, friable embryogenic calluses (75.12%) were induced on half-strength MS (Murashige and Skoog, 1962) basal salts and vitamins, 400mgl−1 of glutamine and 60gl−1 sucrose supplemented with 2.0mgl−1 2,4-D (2,4 dichlorophenoxyacetic acid). Maximum frequency (68.35%) of somatic embryo induction was achieved on embryo induction medium containing half MS basal salts and vitamins, 30gl−1 sucrose and 1.0% activated charcoal. The effects of abscisic acid (ABA), sucrose, and different strengths of MS medium on somatic embryo maturation were also investigated. The highest number of cotyledonary embryos was produced on liquid half-strength MS medium with 10.5mgl−1 ABA and 10.0gl−1 sucrose. Somatic embryos at cotyledonary stage were transferred onto MS medium supplemented with 0.4mgl−1 BAP (6-benzylaminopurine) and 0.02mgl−1 NAA (α-naphthaleneacetic acid) showed regeneration efficiency of up to 62.32%. Plants with well-developed shoots and roots were subsequently hardened in a potting mixture enriched with vermiculite. This regeneration protocol assures a high frequency of somatic embryogenesis, maturation and regeneration into plants.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
