Abstract
Rhizosphere bacteria are one of the most potential biological control agents in the plant disease protection. <i>Bacillus </i>species as a group offer several advantages over other bacteria for protection against pathogens because of their ability to form endospores, and because of the broad-spectrum activity of their antibiotics. Five soil samples from tomato rhizosphere were collected from shambat area, Khartoum State, Sudan. <i>Bacillus</i> isolates were isolated from the rhizosphere of tomato to use as natural bio-control agents. They were screened for antagonism <i>in vitro</i> against <i>Alternaria alternata</i> causal agents of early blight disease of tomato. Serial dilution technique was adopted for the isolation of <i>Bacillus</i> species. Only 27 out of 45<i> Bacillus</i> isolates showed antagonistic properties. Four out of the 27 isolates showed antagonism (Bacillus B25, B35, B41, B45) were identified to the species level by bacteriological assay (morphological and biochemical tests).
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