Abstract
Abstract: Coscinium fenestratum (Gaertn.) Colebr. is an important medicinal plant belonging to the family Menispermaceae. Multiple shoots were formed from epicotyl explants on Murashige and Skoog (MS) medium supplemented with 1.0 μM kinetin (Kin) and 0.25 μM 2,4-Dichlorophenoxy acetic acid (2,4-D). A maximum of five shoots were obtained from one explant in a 75-day culture period. The effect of subsequent subcultures on shoot formation was also studied. Repeated subculture favoured the increase in shoot length and the number of shoots per explant in the media containing Kin and 2,4-D. Higher concentrations of either cytokinin used: butyric acid (BA) or Kin causes stunting of multiple shoots with small and narrow leaves. After 100% in vitr o rooting was obtained in half-strength MS supplemented with 2.5 μM Indole-3-butyric acid (IBA), plantlets were transferred to ex vitro conditions. Following a 15-day in vitro rooting period and 12 days of ex vitro acclimatization, 66.7% of the plantlets were established in the compost beds for another two months to improve the leaf size and then transferred to the field with 100% survival rate. This protocol has the potential for use as a tool for mass scale production of planting material. Keywords: Conservation, Coscinium fenestratum , Menis- permaceae, micropropagation. Doi: 10.4038/jnsfsr.v38i4.2648 J.Natn.Sci.Foundation Sri Lanka 2010 38 (4): 219-223
Highlights
IntroductionCoscinium fenestratum (Gaertn.) Colebr is a woody climber (popularly known as ''weniwel'' in Sinhala) which grows wild in the natural rain forest reserves in Sri Lanka and India
Coscinium fenestratum (Gaertn.) Colebr is a woody climber which grows wild in the natural rain forest reserves in Sri Lanka and India
A) Plant material, surface sterilization and explant isolation: C. fenestratum seeds were collected from wild populations of the Sinharaja rain forest and the Yagirala conservation forest
Summary
Coscinium fenestratum (Gaertn.) Colebr is a woody climber (popularly known as ''weniwel'' in Sinhala) which grows wild in the natural rain forest reserves in Sri Lanka and India. This species is listed as endangered in the IUCN Red Data Book and in the Convention on International Trade in Endangered species of wild Fauna and Flora (CITES) listing because of its large scale harvesting for medicinal use[1]. The objective of this study was to develop a feasible in vitro protocol for mass propagation of C. fenestratum and acclimatize and establish the planting material in home gardens and their natural habitats
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