Abstract

The in vitro production of porcine embryos aims to potentiate oocytes, maximize the use of breeders, create high value and viable embryos for transfer to receivers. This biotechnology involves different steps related to the manipulation of female gametes such ovarian harvesting, follicular puncture, oocyte maturation, oocyte fertilization and embryo culture. Difficulties such as reaching cytoplasmic maturation in the oocytes, high polyspermy ratio as well as the low capacity in embryonic development until the blastocyst stage, and further transfer to synchronized sows are the main reasons why this technique is not yet very popular. However, there has been an effort by the scientific community to improve this technique to move from an experimental level to a field tool.

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