In vitro mutagenicity and genotoxicity of raw and simulated leachates from plastic waste dumpsite

Abstract

Increase in production of different types of plastics has led to increase in the amount of plastic waste generation worldwide. The chemical constituents of these plastic wastes have made their disposal an important economic and environmental health problem globally. This study assessed the mutagenic and genotoxic potential of plastic waste dumpsite raw and simulated leachates using the Ames Salmonella fluctuation test with Salmonella typhimurium strains TA98 and TA100, and the SOS chromotest with Escherichia coli PQ37. Physico-chemical parameters and organic constituents of the leachates were also analyzed. The result of the Ames test showed that the leachates are mutagenic even at low concentration. Also, the TA100 strain was the more responsive strain in terms of mutagenic index in the absence of metabolic activation. The SOS chromotest results complimented the Ames Salmonella fluctuation test results. Nevertheless, the E. coli PQ37 system was slightly more sensitive than the Salmonella assay for detecting mutagens and genotoxins in the tested leachates. Generally, simulated leachate showed a higher mutagenicity and genotoxicity than the raw leachate. Pb, Cd, Cr, Ni, Cu, As, PBDEs, PAHs, PCBs, and Bisphenol A contents analyzed in the leachates were believed to play significant role in the observed mutagenicity and genotoxicity in the microbial assays. These data showed that plastic waste constituents are capable of inducing DNA damage in exposed organisms and might induce similar damage in plants, animals and humans exposed to it, hence, great care should be taken to eliminate indiscriminate disposal of plastics in the environment.