Abstract
Successful tissue culture procedures have been established for rapid multiplication of three interspecific precious hybrids of Vanda. Germination frequency of seeds, protocorm development and seedling growth were qualitatively and quantitatively enhanced by suitably altering the composition of the medium. Among the different growth adjuvants investigated, yeast extract (YE), peptone, urea and naphthaleneacetic acid (NAA) markedly enhanced the growth and differentiation of protocorms. Coconut milk (CM) especially induced the formation of large size seedlings.
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