Abstract
An efficient in vitro propagation protocol was standardized in K. galanga, wherein shoot cultures were raised from rhizome with axillary bud explants in Murashige and Skoog (MS) medium supplemented with different hormonal regimes. The best medium identified for shoot initiation as well as multiplication was MS supplemented with 2 mg/l BAP and 1 mg/l Kin. The high rates of rooting in Half MS media supplemented with 1 mg l−1 IBA and successful transfer to polybags make this production system useful for ex-situ conservation and large scale multiplication of kacholam.
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