Abstract
In vitro effects of heat extracts from firefly squid, Watasenia scintillans, (FSE-1) and desalted FSE-1 (DFSE-1) on the activities of phagocytes and lymphocyte proliferation of rainbow trout were evaluated.In the first experiment, the effects of FSE-1 and DFSE-1 were compared by measuring respiratory burst and potential killing activities of blood phagocytes, and by measuring lymphoblastic transformation level with or without Con A. In this experiment, DFSE-1 was found more effective than FSE-1.In the second experiment, effects of different doses of DFSE-1 were examined by measuring nitro blue tetrazorium (NBT) reduction level, respiratory burst activity and potential killing activity of head kidney macrophages and lymphocyte proliferation test with Con A or LPS. When diluted (t1-t8) DFSE-1 was added, the head kidney macrophages showed significant (pl0.05) elevation of NBT reduction, respiratory burst and potential killing activities compared to the control. The lymphoblastic transformation rate was significantly (pl0.05) higher than that of control when diluted (t1-t16) DFSE-1 was added with Con A or LPS. These results suggest that DFSE-1 administration effectively stimulate the defense mechanism of rainbow trout.
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