Abstract
The wheat germ product Biogerm® was enzymatically digested and subjected to small scale in vitro fermentations with fresh human faeces under anaerobic conditions. Raftiline® and lactulose served each as a control substrate in parallel fermentations. Total gas and short chain fatty acid (SCFA) production from Biogerm® was found to be slower within the first few hours compared to the control substrates, but reached similar end values after 24 h. Moreover, the SCFA pattern of Biogerm® was characterised by high propionate proportions. In order to investigate the effect of Biogerm® on the growth of bifidobacteria, total bacteria and bifidobacteria were enumerated by in situ hybridisation techniques. The wheat germ preparation increased the proportion of bifidobacteria from 15 up to 24% of total bacteria in the faecal matter. Conclusively, Biogerm® has an impact on the growth of bifidobacteria in an in vitro environment containing the human faecal microflora.
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