Abstract
BackgroundThe growing interest on usage of probiotic lactobacilli in maintaining oral health has posed number of questions on its probable side effects. One such consideration could be an increased acid production in dental plaque, in turn leading to dental caries. Thus, the aim of this study was to comparatively evaluate the lactic acid producing ability of L. acidophilus and L. plantarum with and without dental plaque.Material and MethodsThe study consisted of five groups: 3 control groups (Supragingival plaque, L. acidophilus and L. plantarum) and 2 test groups (Supragingival plaque with L. acidophilus and Supragingival plaque with L. plantarum). 26 samples for each group were collected and their baseline spectrophotometric values were recorded. The acid production was initiated by adding 25?l fructose (10%) and stopped by centrifugation for 2 min. The concentration of the lactic acid produced was determined with the aid of COBAS INTEGRA 400 plus.ResultsOn comparison of Lactic Acid estimation in mg/dl, the mean values of Plaque group was the highest followed by Plaque +L acidophilus, Plaque +L plantarum, L acidophilus and least in L plantarum. The posthoc analysis shows that the comparison of Group 1 (Plaque) and Group 2 (Plaque +L acidophilus) is statistically Significant results between all the groups except between the Plaque +L acidophilus and Plaque +L plantarum group.ConclusionsThe lactic acid producing ability of pure suspensions of L.acidophilus and L.plantarum and the lactic acid producing efficiency becomes more when they are added to the supragingival plaque. Key words:Probiotic, lactic Acid, plaque.
Highlights
Dental caries is the most prevailing infectious disease worldwide which results due to the prolonged interaction between microbes and fermentable carbohydrates [1]
Though lactobacillus under normal conditions produces lactic acid as an end product of fermentation process, when it comes in contact with the plaque, it reduces the amount of lactic acid production reducing the cariogenesity
The children who were exposed to food items supplemented with probiotic bacteria, xylitol or any other antimicrobial agents and systemic antibiotics were excluded from the study. -Study groups: The 130 samples selected were randomly divided into five groups: Group 1- Supragingival plaque (Control Group), Group 2- Supragingival plaque with Lactobacillus acidophilus, Group 3- Supragingival plaque with Lactobacillus plantarum, Group 4- Lactobacillus acidophilus (Control Group) and Group 5- Lactobacillus plantarum (Control Group). -Procedure: Children were instructed not to brush their teeth for 24 hours before obtaining plaque samples
Summary
Dental caries is the most prevailing infectious disease worldwide which results due to the prolonged interaction between microbes and fermentable carbohydrates [1]. These are known to cause dental caries as they are excellent acidogenic and aciduric microorganisms. The present study was conducted to comparatively evaluate the lactic acid producing ability of two probiotic strains of lactobacillus namely, L. acidophilus and L. plantarum with and without dental plaque. The growing interest on usage of probiotic lactobacilli in maintaining oral health has posed number of questions on its probable side effects One such consideration could be an increased acid production in dental plaque, in turn leading to dental caries. The aim of this study was to comparatively evaluate the lactic acid producing ability of L. acidophilus and L. plantarum with and without dental plaque. Conclusions: The lactic acid producing ability of pure suspensions of L.acidophilus and L.plantarum and the lactic acid producing efficiency becomes more when they are added to the supragingival plaque
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