Abstract
Abstract An in vitro assay was developed for linoleic acid desaturase activities in chloroplast homogenates using exogenous radiolabelled lipid substrates. Activities were stabilized by addition of catalase to an incubation medium also supplemented with NADPH and BSA. The direct in vitro desaturation of molecular species of MGDG by chloroplast homogenates from soybean and Arabidopsis thaliana was consistent with lipid-linked desaturation proceeding without participation of acyl-CoA intermediates. Linoleic acid was also desaturated in PC substrates. 4-Chloro-5-dimethylamino-2-phenyl-3(2H)-pyridazinone, a herbicide known to reduce levels of linolenic acid in certain plant membrane lipids, directly inhibited in vitro desaturation of fatty acids in both MGDG and PC substrates.
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