Abstract
Third-stage (L3) Contracaecum sp. larvae, from Gadus morhua, developed to sexual maturity in a two-step roller culture system using medium API-1 supplemented with 5 mM L-cysteine only (step 1) and with bovine haem (Fildes' reagent) added (step 2). The gas phase was 85% N2 – 10% CO2 – 5% O2 and the culture was maintained at 35 °C. The mature nematodes proved to be Contracaecum osculatum, a species which normally matures in the stomach of pinnipeds.
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