Abstract
Introduction: Root Canal Filling is the final procedure during root canal therapy, where endodontic sealers should have physical, chemical, and biological properties that help to reduce the population of residual bacteria and create a favorable environment for the repair of periapical tissues. The aim of this work was to compare the antibacterial capacity of AH Plus (Denstply®) and BioRoot RCS (Septodont®). Materials and Methods: Negative-Coagulase Streptococcus, Streptococcus salivarius, and Enterococcus faecalis were isolated from a necrotic pulp. They were tested by Minimum Inhibitory Concentration (MIC): 50 µl of saline solution and 100 µl of AH Plus and BioRoot RCS were used to perform the following dilutions of 1:2, 1:4, 1:8, 1:16, 1:32, 1:64, 1:128, 1:256, 1:512, 1:1024. Subsequently, the samples were cultured on Müeller- Hinton agar with sheep blood, to assess bacterial growth. Results: AH Plus showed growth up to 1:64 dilution and BIOROOT = 1:32 in E. faecalis while in Negative-Coagulase Streptococcus both cements showed growth at 1:2048 dilution, in S. salivarius there was no bacterial growth with both cements. Conclusion: AH plus (Denstply®) and BioRoot RCS RCS (Septodont®) are cements that provided an effective bacterial activity in isolated microorganisms: E. faecalis, S. Coagulase Negative and S. salivarius.
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