In vitro bionics of face centered cubic lattice crystal nanoparticles by Saccharomyces cerevisiae and its microbicidal screening

Abstract

Extracellular synthesis of nanoparticles has received attention due to its more advantageous form of synthesis in large quantities and also easy for downstream processing. In the present investigation, we report in vitro extracellular synthesis of silver nanoparticles (AgNPs) using Saccharomyces cerevisiae with silver nitrate solution. The AgNPs were produced in 72 h of incubation time. The AgNPs formed were further characterized by means of UV-Vis spectrophotometry, Fourier Transform Infra-Red (FTIR), X-Ray Diffraction (XRD), and Scanning Electron Microscope (SEM). The synthesized AgNPs had maximum absorbance at ∼420 nm in the UV-visible region. FTIR bands were observed at 3353.40, 2075.09, 1637.71 and 1397.30 cm−1. XRD patterns of the AgNPs confirmed the formation of face centered cubic (fcc) lattice crystal silver particles. The SEM analysis revealed that the size of the AgNPs were of 30–60 nm. The synthesized AgNPs inhibited the growth of the test microorganisms at the concentration of 100 μL. The present study highlights the possibility of using the common baker’s yeast S. cerevisiae to synthesize AgNPs and as a microbicidal agent.