Abstract
This study aimed to evaluate in vitro the antimicrobial effect of a bioadhesive chitosan-based oral membrane with chlorhexidine for local treatment of infections in the oral tissues. Five oral membranes of different compositions were tested: 5% chitosan (G1); 5% chitosan ± 0.2% chlorhexidine (G2), 5% chitosan ± 0.6% chlorhexidine (G3), 5% chitosan ± 1.0% chlorhexidine (G4), and 5% chitosan ± 2.0% chlorhexidine (G5). Also, five gel types were tested according to the following compositions: 5% chitosan gel (G6), 0.2% chlorhexidine gel (G7), 2.0% chlorhexidine gel (G8), 5% chitosan gel ± 0.2% chlorhexidine gel (G9), and 5% chitosan gel ± 2.0% chlorhexidine gel (G10). The antimicrobial action of the samples was tested against Candida albicans and Streptococcus mutans through antibiogram by measuring the inhibition halos. Data were statistically analyzed by Kruskal-Wallis and one-way ANOVA followed by Tukey test (p<0.05). The 2.0% chlorhexidine membrane (G5) and the disks containing 2.0% chlorhexidine gel (G8) showed the greatest inhibition halos for both microorganisms, with statistically significant difference when compared to others tested groups (p=0.008) only for Candida albicans inhibitions results. All the other formulations of membranes and gels showed inhibition halos, but without statistically significant difference. The bioadhesive chitosan-based oral membrane with 2% chlorhexidine and 2% chlorhexidine gel were the most effective in inhibiting the tested microorganisms.
Highlights
Dentistry requires therapeutic alternatives to be applied on surgical wounds and wounds caused by physical and/or chemical trauma, periodontal and dental pulp infections, and other types of ulcerated oral lesions [1,2,3,4,5]
This study aimed to evaluate in vitro the antimicrobial effect of a bioadhesive chitosan-based oral membrane with chlorhexidine for local treatment of infections in the oral tissues
The inhibition halo formed for each oral membrane
Summary
Dentistry requires therapeutic alternatives to be applied on surgical wounds and wounds caused by physical and/or chemical trauma, periodontal and dental pulp infections, and other types of ulcerated oral lesions [1,2,3,4,5]. The development of an alternative vehicle to allow the releasing and the contact of the drug with the affected tissue, for a longer time, will greatly contribute to repair [6,7,8]. Biomaterials allow interaction and repair of a biological system [1,9,10]. Key features such as biodegradability and biocompatibility are fundamental for new biomaterials to be developed in medical and dental area [5,6,11,12]. Wieckiewicz et al [16] affirmed it can be applied in all fields of dentistry including preventive dentistry, conservative dentistry, endodontics, surgery, periodontology, prosthodontics and orthodontics
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