Abstract
We evaluated the antibacterial activity of three diterpenes isolated from natural sources against a panel of microorganisms responsible for bovine mastitis. ent-Copalic acid (CA) was the most active metabolite, with promising MIC values (from 1.56 to 6.25 µg mL−1) against Staphylococcus aureus (ATCC and clinical isolate), Staphylococcus epidermidis, Streptococcus agalactiae, and Streptococcus dysgalactiae. We conducted time-kill assays of CA against S. aureus, a commensal organism considered to be a ubiquitous etiological agent of bovine mastitis in dairy farms worldwide. In the first 12 h, CA only inhibited the growth of the inoculums (bacteriostatic effect), but its bactericidal effect was clearly noted thereafter (between 12 and 24 h). In conclusion, CA should be considered for the control of several Gram-positive bacteria related to bovine mastitis.
Highlights
Bovine mastitis is an inflammatory condition that affects the mammary glands of cows
Considering the growing need for new antibacterial agents that can be used to control and treat bovine mastitis and as part of our ongoing efforts to explore the antibacterial properties of diterpenes, here we investigate the antimicrobial activity of MO, kaurenoic acid (KA), and Copalic acid (CA) against a representative panel of bacteria responsible for this pathology
The antimicrobial effects of MO, KA, and CA were evaluated against a panel of American Type Culture Collection (ATCC) and clinically isolated bacteria associated with bovine mastitis
Summary
Bovine mastitis is an inflammatory condition that affects the mammary glands of cows It has a significant impact on animal production, animal welfare, and milk quality [1]. Natural products are a rich and promising source for the discovery of new biologically active compounds [4]. Our research group has investigated the antibacterial activity of naturally occurring diterpenes against bacterial strains responsible for human pathologies like caries, periodontitis, pneumonia, and nosocomial infections, among others. Considering the growing need for new antibacterial agents that can be used to control and treat bovine mastitis and as part of our ongoing efforts to explore the antibacterial properties of diterpenes, here we investigate the antimicrobial activity of MO, KA, and CA against a representative panel of bacteria responsible for this pathology
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