Abstract
Anti-inflammatory and in vitro cytotoxic effect of phenols of Hermannia geniculata (PoHG) on Vero and HepG2 cells was carried out using Soybean lipoxygenase and MTT assays. PoHG extract exhibited a commendable inhibition of 5-lipoxygenase enzyme with IC50 value of (150 ± 0.03) µg/mL which is similar to the IC50: (110± 0.01) µg/mL of the standard (indomethacin). However, the extract was non-toxic to Vero cells with LC50 value >1.00 mg/mL but highly toxic to HepG2 cells (LC50: 0.05 mg/mL). A decrease viability of HepG2 cells was observed with increase in the concentration of the extract. There was less than 5% viable HepG2 cells at PoHG concentration of 750 µg/mL. The selectivity index of (20.00 and 33.33) was recorded for PoHG extract and doxorubicin respectively. The anti-inflammatory activities of PoHG suggested that the phenols extract may be useful in the management of inflammatory diseases like artheriosclerosis, diabetes mellitus, rheumatoid arthritis and asthma. It is also safe for use while its antiproliferative activities can be exploited in search for anticancer agents.
Highlights
Inflammation is defined as the body response to injury
phenols of Hermannia geniculata (PoHG) extract exhibited a commendable inhibition of 5lipoxygenase enzyme with IC50 value of (150 ± 0.03) μg/mL which is similar to the IC50: (110± 0.01) μg/mL of the standard
The result obtained from this study shows commendable inhibition of 5-lipoxygenase enzyme by PoHG extracts
Summary
Inflammation is defined as the body response to injury. Injury may result from exogenous sources including invasion of ectoparasites, viruses, bacteria and fungi while endogenous injury may result from endoparasites, defective immune system, hypersensitivity and anaphylactic reactions [1, 2]. The development of chronic inflammation play key role in different pathological conditions like insulin resistance, ulcer, diabetes, arthritis and asthma [3]. Anti-inflammatory mediators play key role in the process of inflammation resolution [4]. Africa is blessed with several medicinal plants that has anti-inflammatory activity. H. geniculata is among the medicinal plant species frequently used in South Africa for the management of different diseases [8,9,10]. It belongs to the genus of flowering plant from the family Malnaceae. This study was design to test the anti-inflammatory properties of PoHG and determine its cytotoxic effect on Vero and HepG2 cells in order to determine its safety and possible antiproliferative activities
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