In-Vitro Anti Cancer Activity Of Irinotecan & Sunitinib Loaded Nanobubbles On Selected Cell Lines.

Abstract

The objective of the present research is to develop, analyze, and evaluate the cytotoxic effects of nanobubbles loaded with irinotecan and sunitinib for colorectal cancer therapy.Drug loaded dextran sulfate nanobubbles were formulated using the emulsification technique and the prepared nanobubbles were evaluated for qualitative and quantitative parameters. Cell viability MTT assay was performed to evaluate the irinotecan and sunitinib loaded nanobubblesfor cytotoxicity or ability to inhibit cell proliferation spectrophotometrically as a function of mitochondrial activity in living CR4 and A-549(irinotecan), CR4 and A-498 (sunitinib) cell lines.The irinotecan and sunitinib loaded nanobubbles were prepared successfully and the evaluated qualitative and quantitative parameters were within in the acceptable range. The in vitro anti colorectal cancer activity of irinotecanperformed on CR4 and A-549 cell lines and IC50 values are found 70.41 and 73.26 µg/ml, respectively, where activity of sunitinib performed on CR4 and A-498 cell lines and IC50 values are found to be 84.34 and 60.08 µg/ml, respectively which show excellent anticancer activity.This result concludes that the prepared irinotecan and sunitinib nanobubbles were prepared with accepted characterizations and have almost no cytotoxic effects on the CR4, A-549, A498 cell lines.