Abstract
Immunoreactive (IR) prostaglandin H synthase (PGHS) was evident in primary cortical cultures as early as day 2 after seeding. Labeling did not increase with time in culture, nor was there an apparent difference in IR intensity between 2 and 10% serum cultures or between glial and neuronal figures. PGHS-1 IR appeared as a homogeneous cytoplasmic fluorescence compared with PGHS-2 IR which tended to be more intense, particulate and exclusively perinuclear. PGHS-2(+) IR in both neurons and glia increased with time in culture. Immunofluorescence varied in intensity, but no significant degree of variation was seen between cell types. Neuronal PGHS-2 IR extended into processes and amassed in growth cones and at the leading edge processes of astrocytes. Novel rosette formations, possibly lipid bodies, were common in cultured neurons, but not astrocytes.
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