Abstract
The current knowledge of in vitro adherence of Staphylococcus pseudintermedius to canine corneocytes is limited to comparative analyses between strains, staphylococcal species or corneocytes collected from different breeds, body sites and hosts. However, the role played by colonization status of corneocyte donors remains unknown. The aim of this study was to evaluate the adherence properties of commensal S. pseudintermedius strains to corneocytes collected from dogs with different colonization status. For this purpose, corneocytes were collected from five dogs that were classified as persistently colonized (D1 and D2), intermittently colonized (D3 and D4) or non-colonized (D5) on the basis of the results of a previous longitudinal study. Adherence to corneocytes originating from each of the five dogs was assessed by an in vitro adhesion assay using four genetically unrelated strains isolated from the colonized dogs (S1 to S4). Irrespective of their host of origin, all strains adhered significantly better to corneocytes from D1 and D2 than to corneocytes from D3, D4 and D5 (P < 0.0001). The mean count of cells adhering to corneocytes from persistently colonized dogs was on average three times higher than the mean count using corneocytes from the other dogs. A significant difference between strains was only observed for one strain-corneocyte combination (S2-D4), indicating that S. pseudintermedius adherence to corneocytes is driven by host factors and only marginally influenced by strain factors. This finding has important implications for understanding and preventing S. pseudintermedius skin colonization and infection.
Highlights
Staphylococcus pseudintermedius is an opportunistic pathogen that resides on the skin and mucosae of dogs and other members of the Canidae
The mean count of bacteria adhering to corneocytes from D1 and D2 was 224 cells per microscope field (95% confidence interval: 193–258), whereas the mean count using corneocytes from non-persistent carriers was only 74 cells per field (95% confidence interval: 61–89)
The results indicate a greater adhesion of S. pseudintermedius to corneocytes from persistent carriers than intermittent carriers or non-carriers, regardless if the strain was resident or non-resident in the dog from which the corneocytes were collected
Summary
Staphylococcus pseudintermedius is an opportunistic pathogen that resides on the skin and mucosae of dogs and other members of the Canidae. We have previously shown that most dogs are either persistent or intermittent carriers, and very few individuals are non-carriers over a period of one year [2]. Staphylococcal adherence to corneocytes is a crucial step for skin colonization and subsequent infection. Previous considered the possible role played by the colonization status of the dogs from which corneocytes were collected. In order to fill this knowledge gap, the aim of this study was to evaluate the adherence properties of commensal S. pseudintermedius strains to corneocytes collected from dogs with different colonization status. In vitro adherence was assessed using strains and corneocytes collected from persistently colonized, intermittently colonized and non-colonized dogs from a previous longitudinal study
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