Abstract
AbstractPlasma‐free protein manufacturingGrillberger, XX, et al., Biotechnol. J. 2009, 4, 186–201Mammalian cells are the expression system of choice for therapeutic proteins, especially those requiring complex post‐translational modifications. Traditionally, these cells are grown in medium supplemented with serum to support viability and productivity. However, due to regulatory concerns about the inherent potential for transmission of infectious agents as well as the heterogeneity and lack of reliability of the serum supply, a trend has emerged to eliminate the use of plasma‐derived additives in the production and formulation of recombinant protein therapeutics. This review by researchers from Baxter (Austria) describes the development and examples of advances in manufacturing that have made possible the removal of human and animal‐derived products from all steps of recombinant protein production.Inkjet‐printed immunoassayStewart, XX, et al., Biotechnol. J. 2009, 4, 206–209The development of a cost‐effective method for manufacturing immunoassays is a key step towards their commercial use. In this study by researchers from North Carolina (NC, USA), a piezoelectric inkjet printer and a nylon membrane were used to fabricate a disposable immunoassay. After testing with horseradish peroxidase and tetramethylbenzidine precipitates they show a cross‐hatch pattern of functional antibodies in uniform patterns. These results suggest that piezoelectric inkjet printing may be used to fabricate low‐cost disposable immunoassays for biotechnology and healthcare applications.Miniaturized cell lysisLin and Cai, Biotechnol. J. 2009, 4, 210–215Cell lysis, performed to disrupt the cell wall structure to release the intracellular contents, is a routinely used technique in the field of biology, for example, in extraction of cellular products from microorganisms, protein or enzyme screening, clinic diagnosis and bioanalytical detection of mammalian cells. Widely and routinely used cell lysis techniques are still being evolved to address the technical needs of microscale sample processing, for example in high‐throughput screening or miniaturized assays. This Technical Report by researchers from Beijing (China) focuses on several recently emerged mechanical, physical and biological methods designed for lysing cells on small scales for screening or analysis.
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