In silico approach to develop structure and functional analysis of response receiver regulator protein of the strain pseaudomonas fulva 12x

Abstract

In total world population, most of the people depend on rice as a staple food because it gives proper amount of nutrition, which help to people in leading healthy life mainly in the developing countries of Asia. This valuable plant is attacked by various types of micro-organism like bacteria. Under the pseaudomonas species, pseaudomonas fulva 12-x is one of the strain, contain receiver domain include regulator protein, which ultimately help them to maintain signal transduction pathway.so the aim is to find out new target site in this hazardous bacteria to develop a potential medicine that help in saving our crops without using fertilizer because fertilizer causes various types of environmental problem. Very little amount of information is known about the structural biology of pseaudomonas fulva 12-x,so using homology modeling of bioinformatics approach the tertiary structure of response receiver regulator protein of pseaudomonas fulva 12-x has determined. The model building was done by swiss model workspace. Then the model was evaluated and validated using PROCHEK, Verify 3d,PROSA and ERRAT in these the verify 3d value was 0.70 suggest surrounding profile is good an (PROSA) determined the disallowed region contain 0.0% and allowed region contain 93.5% and most favored region contain 6.5% amino acid, which implies perfect sterochemical quality of the model. Then physico-chemical characterization, secondary structure and functional site prediction were determined using Expasys protparam server, GOR4, SOPMA and Scan prosite tools. Finally, the active site was analyzed using CASTp server, which predicted target region and help in developing medicine for various rice diseases like bacterial PANICLE BLIGHT disease in further lab test.