Abstract
Degradation of insulin was studied in this work. Casein and protamine could obviously suppress degradation of insulin by intestinal enzymes, and could protect insulin from degradation by the mechanism of competition and combination with proteolysis enzyme. What is more, co-incubated with HP-β-CD-casein or HP-β-CD-protamine, most insulin was protected from degradation by intestinal enzymes. In addition, it was found that the complexation of insulin with HP-β-CD was characterized by UV absorption spectra. These results indicated that HP-β-CD, casein and protamine could offer some positive and useful results, and could protect insulin from degradation during their transit through the intestinal tract.
Highlights
Insulin has been in therapeutic use for almost 80 years, it has been the most effective and durable drug in the treatment of advanced-stage diabetes [1]
Effects of HP-β-CD and casein on degradation of insulin induced by α-chymotrypsin α-Chymotrypsin is a common protease in the intestine, so degradation of insulin by α-chymotrypsin was investigated in vitro
In order to examine whether HP-β-CD and casein could protect insulin from degradation or not, HP-β-CD and casein was examined, respectively
Summary
Insulin has been in therapeutic use for almost 80 years, it has been the most effective and durable drug in the treatment of advanced-stage diabetes [1]. Despite significant advancements in the pharmaceutical research field, development of a proper non-invasive insulin delivery system remains a. The oral route represents the most convenient way of drug administration due to high patient compliance and comfort. One of the most important characteristics of oral delivery of peptides and proteins is the need to protect these drugs from proteolytic attack during their transit through the gastrointestinal (GI) tract [5]. Enzyme inhibitors show several safety problems for clinical applications, a safe and convenient way which can protect insulin from degradation by enzymes in the intestines is an important aspect to be considered
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