Improving Donor Programs With An Oocyte Bank Using Vitrification

Abstract

ObjectiveThe use of oocytes for a regular donor program can have same inconveniences like a waiting list and synchronization of patients. The storage of human oocytes can avoid those problems. The storage of human oocytes could be indicated in women that undergo oncological treatments, at risk to loose their ovarian function or simply in those that want to delay the motherhood. The aim of this work is to show our encouraging data from our vitrified oocytes and suggest the possibility to run oocyte banks by vitrification to improve the donor programs.DesignProspective study.Materials and methodsFrom July 2004 to April 2005, we analyzed the survival, fertilization, embryo cleavage, implantation, preg-nancy and abortion rates from 60 vitrified oocytes, obtained from our donor patients and frozen by vitrificaction. The oocytes were stored and thawed when the recipient ask for the donation program.ResultsAfter thawing 60 vitrified oocytes, 46 survived (76.60%), after ICSI 38 oocytes fertilized (82.60%) and 34 embryos developed normally (cleavage rate of 89.47%). We transfer all embryos in to a 10 recipients patients and get 8 pregnancies (80%): 5 singleton (62.5%) and 3 multiple (37.5%), with an implantation rate of 32.3% . The abortion rate was 50% (4 patients) and the ongoing pregnancy is 50% (4 patients: 3 singletons and 1 twin).ConclusionThe vitrification technique has demonstrated to be an effective and easy method for freezing oocytes with excellent survival, embryo development and pregnancy rates, and could be a good alternative to run oocyte Banks to improve donor programs. ObjectiveThe use of oocytes for a regular donor program can have same inconveniences like a waiting list and synchronization of patients. The storage of human oocytes can avoid those problems. The storage of human oocytes could be indicated in women that undergo oncological treatments, at risk to loose their ovarian function or simply in those that want to delay the motherhood. The aim of this work is to show our encouraging data from our vitrified oocytes and suggest the possibility to run oocyte banks by vitrification to improve the donor programs. The use of oocytes for a regular donor program can have same inconveniences like a waiting list and synchronization of patients. The storage of human oocytes can avoid those problems. The storage of human oocytes could be indicated in women that undergo oncological treatments, at risk to loose their ovarian function or simply in those that want to delay the motherhood. The aim of this work is to show our encouraging data from our vitrified oocytes and suggest the possibility to run oocyte banks by vitrification to improve the donor programs. DesignProspective study. Prospective study. Materials and methodsFrom July 2004 to April 2005, we analyzed the survival, fertilization, embryo cleavage, implantation, preg-nancy and abortion rates from 60 vitrified oocytes, obtained from our donor patients and frozen by vitrificaction. The oocytes were stored and thawed when the recipient ask for the donation program. From July 2004 to April 2005, we analyzed the survival, fertilization, embryo cleavage, implantation, preg-nancy and abortion rates from 60 vitrified oocytes, obtained from our donor patients and frozen by vitrificaction. The oocytes were stored and thawed when the recipient ask for the donation program. ResultsAfter thawing 60 vitrified oocytes, 46 survived (76.60%), after ICSI 38 oocytes fertilized (82.60%) and 34 embryos developed normally (cleavage rate of 89.47%). We transfer all embryos in to a 10 recipients patients and get 8 pregnancies (80%): 5 singleton (62.5%) and 3 multiple (37.5%), with an implantation rate of 32.3% . The abortion rate was 50% (4 patients) and the ongoing pregnancy is 50% (4 patients: 3 singletons and 1 twin). After thawing 60 vitrified oocytes, 46 survived (76.60%), after ICSI 38 oocytes fertilized (82.60%) and 34 embryos developed normally (cleavage rate of 89.47%). We transfer all embryos in to a 10 recipients patients and get 8 pregnancies (80%): 5 singleton (62.5%) and 3 multiple (37.5%), with an implantation rate of 32.3% . The abortion rate was 50% (4 patients) and the ongoing pregnancy is 50% (4 patients: 3 singletons and 1 twin). ConclusionThe vitrification technique has demonstrated to be an effective and easy method for freezing oocytes with excellent survival, embryo development and pregnancy rates, and could be a good alternative to run oocyte Banks to improve donor programs. The vitrification technique has demonstrated to be an effective and easy method for freezing oocytes with excellent survival, embryo development and pregnancy rates, and could be a good alternative to run oocyte Banks to improve donor programs.