Abstract
To examine the effects of human amniotic fluid stem cells (hAFSCs) transplantation on bladder function and molecular changes in diabetic rats, 60 female Sprague-Dawley rats were used for study. Three groups were assigned including sham control rats, streptozotocin (STZ, 60 mg/kg)-induced diabetic rats and STZ-induced diabetic rats plus bladder hAFSCs transplantation. Compared to controls, diabetic rats had decreased body weight but increased bladder weight. Cystometries showed non-voiding contraction, residual volume, voided volume and intercontraction interval increased significantly in diabetic rats at week 4 and 12 after DM induction, but improved after hAFSCs transplantation. The immunoreactivities and mRNAs of nerve growth factor (NGF) decreased significantly in diabetic bladder at week 4 and 12 after DM induction, but recovered after hAFSCs transplantation. The immunoreactivities and mRNAs of M2 and M3 muscarinic receptor increased significantly in diabetic bladder at week 4 after DM induction but recovered after hAFSCs transplantation. The immunoreactivity of 8-hydroxy-20-deoxyguanosine increased significantly in diabetic bladder at week 4 and 12 after DM induction but reduced after hAFSCs transplantation. The present study showed bladder dysfunction in STZ-induced diabetic rats could be improved by hAFSCs transplantation into bladder, which may be related to the recovery of bladder NGF and muscarinic receptors.
Highlights
Diabetes mellitus (DM) is a metabolic disease with multiple serious complications including retinopathy, neuropathy and nephropathy[1,2]
We investigated the effects of human amniotic fluid stem cells transplantation to improve bladder function and molecular changes using a STZ-induced diabetic rat model
Bladder dysfunction subsequent to DM induction is improved after human amniotic fluid stem cells (hAFSCs) transplantation
Summary
Diabetes mellitus (DM) is a metabolic disease with multiple serious complications including retinopathy, neuropathy and nephropathy[1,2]. Oxidative stress has been reported to be associated with the development of diabetic cystopathy in streptozotocin-induced diabetic rats[6,7]. The role of oxidative stress in diabetic bladder dysfunction remains to be clarified. Cellular therapy has been conducted for bladder dysfunction, but only few studies have been reported such as the use of adipose tissue-derived stem cells to improve diabetic bladder dysfunction in streptozotocin (STZ) induced diabetic rats[8,9]. Adult stem cells from adipose tissue have the disadvantages of restricted differentiation potential and a shorter life span compared to amniotic fluid stem cells[10]. We investigated the effects of human amniotic fluid stem cells (hAFSCs) transplantation to improve bladder function and molecular changes using a STZ-induced diabetic rat model
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