Abstract
With 1 figure and 7 tablesAbstractThe current study aims at producing doubled haploid Brassica rapa plants through isolated microspore culture. Genotypic differences in embryogenic response and regenerative ability of microspore‐derived embryos to plants were observed. Compared with the longer incubation period (72 h), heat shock treatment at 33°C for 24 or 48 h was more beneficial for microspore embryogenesis. Microspore embryogenesis was improved with the addition of 6‐benzylaminopurine and naphthalene acetic acid to the NLN medium. The highest frequency of microspore embryogenesis was achieved in ‘SH‐8’ (21.1 embryos/bud), whereas ‘SH‐6’ had the best regeneration ability (67.4%). In total, 1112 regenerated plants were produced from 2025 embryos in a series of experiments using different modifications of microspore culture technique. The average spontaneous doubling frequency of the different genotypes of B. rapa was 57.5%.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
