Abstract

A method is described for the gas-liquid chromatographic separation and estimation of the following compounds obtained by the saponification of ester linkages present in plant lignins: p-coumaric acid, ferulic acid and vanillin. A similar method is described for the separation and quantitative estimation of the following compounds produced by alkaline nitrobenzene or alkaline cupric oxide oxidation of plant lignin: p-hydroxybenzaldehyde, vanillin, syringaldehyde, p-hydroxyacetophenone, acetovanillone and acetosyringone. p-Coumaric and ferulic acids were identified in the oxidation products of grass lignin. As little as 1 μg of a compound could be quantitatively estimated by these methods.

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