Abstract
In-gel competitive reassociation (IGCR) technique is one of the DNA subtraction methods with a high capacity. We have improved the IGCR technique and overcame several disadvantages such as time-consuming and complex process with poor reproducibility. The improved IGCR method was applied to the analysis of human gastric adenocarcinoma genomic DNA. The genomic DNA library, which was constructed after the subtraction by IGCR of a normal gastric tissue genomic DNA from an adenocarcinoma genomic DNA in the same patient, contained 13.9% clones of Epstein-Barr virus (EBV) genomic DNA fragment, which is known to be a cause of Burkitt lymphoma. The quantification of EBV genomic DNA revealed that the detection of these EBV fragments originated in the gastric adenocarcinoma genomic DNA was due to the condensation from a few copies to about 100 thousand copies by the improved IGCR technique. This suggests that our improved IGCR technique can efficiently enrich differences between two genomic DNAs with high simplicity.
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