Abstract
to evaluate the microbial load and adherence of Escherichia coli in different areas of the surgical instrument surface exposed to experimental contamination over time. experimental study in which fragments of crile forceps (serrated, rod and rack) were contaminated by immersion in Tryptic Soy Broth, containing 106 CFU/mL of E. coli, for 1, 2, 4, 6, 8, 12 and 24 hours. Microbial load and bacterial adherence were evaluated using microbiological culture and scanning electron microscopy, respectively. there was an increase in the microbial load on the surgical instrument, proportional to the contamination interval, ranging from 102 after 1 hour to 105 CFU/cm2 in 24 hours. The presence of exopolysaccharide was detected after two hours of contamination. microbial load and adhesion of E. coli increased over time, reaching 105 CFU/cm2 after 24 hours of contamination, starting biofilm formation after two hours.
Highlights
METHODSEvery year, millions of surgeries are performed, and this number is growing[1]
As these are medical devices that can be processed, the surgical instrument must undergo a series of sequential and interdependent actions that include cleaning, drying, evaluation of integrity and functionality, preparation and sterilization, in order to allow its safe reuse[7]. Among these actions, cleaning is pointed as a critical point, because, when properly performed, it promotes the removal of dirt, aiming at reducing the levels of microorganisms, endotoxins, proteins and blood, a fundamental condition to reach the sterility of the surgical instrument after processing[8,9]
The same happened in the following interval, when the bacterial loads reached 104 CFU/cm2, with six hours, being this average maintained until the time of eight hours, except for the shank fragment, in which there was a reduction to the average value of 103 CFU/cm2
Summary
METHODSEvery year, millions of surgeries are performed, and this number is growing[1]. Surgical site infection is a multifactorial adverse event, highlighting the use of the sterile instrument, free of contamination, as one of the factors that can contribute to greater safety of the procedure depending on the microbial load and virulence, the type of procedure and the immune response of the patient[4,5,6]. As these are medical devices that can be processed, the surgical instrument must undergo a series of sequential and interdependent actions that include cleaning, drying, evaluation of integrity and functionality, preparation and sterilization, in order to allow its safe reuse[7]. Among these actions, cleaning is pointed as a critical point, because, when properly performed, it promotes the removal of dirt, aiming at reducing the levels of microorganisms, endotoxins, proteins and blood, a fundamental condition to reach the sterility of the surgical instrument after processing[8,9]
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