Abstract
This study evaluated osmotic shock pre-treatment, a solvent-free wet lipid extraction method, on two lipid-rich microalgal species, Dunaliella salina and Chaetoceros muelleri. The biogas potential of the lipid-spent microalgae was evaluated to assess the suitability of an integrated biorefinery of biodiesel and biogas production. The obtained results revealed the high potential of the diatom C. muelleri for biofuels production when silica starvation is applied at the final stages of the cultures. The osmotic shock had a higher impact on C. muelleri than on D. salina, with a lipid recovery efficiency of 72% and 21% respectively. Besides the high percentage of lipids recovered with this method for C. muelleri, the lipid-spent biomass showed ones of the highest methane yields reported for microalgae, 484 mL CH4 g VS−1. Overall, these results indicate that C. muelleri could be a target species for combined biodiesel and biogas biorefinery.
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