IMPACT OF INSULIN RESISTANCE ON CARDIAC AND RENAL CHANGES ASSOCIATED WITH HIGH SODIUM DIET IN RATS: PP.29.144

Abstract

The objective of this study was to evaluate the impact of fructose-induced insulin resistance on cardiac and renal changes induced by an early and chronic high sodium intake in Sprague- Dawley rats. Immediately after weaning, rats were fed with the normal salt diet (NS, 0.64% NaCl, or a high salt diet (HS, 8% NaCl) for 20 weeks (n = 16 for each diet). At 8 weeks, half of the rats received a diet enriched in fructose (HF, 60%) with the same contain of NaCl (NS or HS) for 12 weeks. At the end of diet, tail-cuff and arterial pressures were measured in conscious rats. Glucose metabolism was assessed by intra-peritoneal glucose tolerance test and glomerular filtration was estimated by creatinin clearance (Cr Cl). Urinary albumin excretion (UAE) was measured by ELISA test. Heart and kidney weight index (HWI and KWI respectively) were determined. High sodium intake induced a slight increase in blood pressure, a marked increase in cardiac and renal mass, albuminuria and an alteration of renal function but had no influence on glucose metabolism. High fructose intake was associated with a rise in cardiac and renal mass, a decrease in renal function independently of blood pressure and an insulin resistance without glucose intolerance. In high salt fed rats, high fructose diet induced insulin resistance, slightly reduced blood pressure and cardiac mass but did not influence renal changes. Subsequent high fructose intake did not worsen cardiac and renal alterations induced by high salt feeding despite the induction of insulin resistance.