Abstract
Bacteria Encoding a Major House-dust Mite Allergen P. Rigaux1, C. Daniel2, C. Ratajczak3, B. Pot2, J. Pestel3, A. Jacquet1; 1Service de Genetique Appliquee, Universite Libre de Bruxelles, Gosselies, BELGIUM, 2Laboratoire de Bacteriologie des Ecosytemes, Institut de Biologie de Lille, Lille, FRANCE, 3Inserm U416, Institut Pasteur de Lille, Lille, FRANCE. RATIONALE: Experimental human and animal studies demonstrated that lactic acid bacteria (LAB) strains have beneficial effects on allergy. This study evaluated some immunomodulatory properties of the Lactobacillus plantarum strain producing a recombinant form of Der p 1 allergen. METHODS: The coding cassette for the MBP-Pro Der p 1 fusion protein was inserted into an expression vector suitable for the allergen production in the LAB used. Human monocyte-derived dendritic cells (MD-DCs) from healthy donors were stimulated with the wild type or recombinant bacteria. Cell surface markers expression (CD80, CD86, CD40, HLADR) and cytokine production (IL-12, IL-10) were analysed by FACS and ELISA respectively. Stimulated DCs were further co-cultured with naive or memory autologous T cells and lymphoproliferation and cytokine production (IFN, IL-4, IL-5) determined. RESULTS: A recombinant LAB producing intracellularly large amounts of MBP-ProDer p 1 was obtained. This strain induced as wild type a dosedependent cytokine production by DC (IL-12 and IL-10), upregulated the expression of costimulatory molecules (CD80, CD86 and CD40) and the maturation marker HLA-DR. Interestingly, in both cases stimulated DCs induce the lymphoproliferation of T naive or T memory cells and favour the production of IFNinstead of Th2 cytokines (IL-4 and IL-5). CONCLUSIONS: As recombinant L. plantarum has similar strong immunomodulatory properties as wild type strain its potential regulatory effect to limit house dust mite allergy appears promising. Funding: Fonds pour la formation a la Recherche dans l’Industrie et dans l’Agriculture (F.R.I.A.)
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