Abstract
Four types of carbamyl phosphate synthetase have been previously distinguished on the basis of catalytic properties and metabolic role. Immunoblot assay has now demonstrated cross-reactivity between rat liver carbamyl phosphate synthetase I and the following other three types of synthetases: carbamyl phosphate synthetase II from SV40-transformed baby hamster kidney cells, carbamyl phosphate synthetase III from spiny dogfish liver and from largemouth bass liver, and Escherichia coli carbamyl phosphate synthetase. The strongest cross-reactivity was observed between carbamyl phosphate synthetases I and III. These findings indicate at least partial structural homology among the various synthetases and constitute the first demonstration of such a relationship among the enzymes.
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