Abstract
Immunolocalization of K AB−2 (Kir4.1), an inwardly ectifying K + channel with a putative ATP-binding domain, was examined in rat kidney where expression of K AB−2 mRNA was previously shown. Anti-K AB−2 antibody was raised in rabbit and then affinity-purified. An immunohistochemical study revealed that K AB-2 immunoreactivity was detected specifically in the basolateral membrane of distal tubular epithelia. Therefore, K AB-2 is the first K + channel shown to be localized in the basolateral membrane of renal epithelia. The finding suggests that K AB-2 may contribute to supplying K + to the Na +-K + pump, which is abundant in the basolateral membrane of distal tubular epithelia, as well as to maintenance of the deep negative membrane potential of these cells.
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