Abstract
Summary: Antisera against the Calvin cycle enzymes d-ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) and phosphoribulokinase (PRK) have been used in immunogold electronmicroscopy studies on cell sections of the cyanobacterium Chlorogloeopsis fritschii. RuBisCO antiserum consistently labelled the carboxysomes (polyhedral bodies) and the cytoplasm. PRK antiserum-labelling occurred in the cytoplasm but not in the carboxysomes. The data agree with in vitro enzyme localization studies, and confirm that both enzymes occur in the cytoplasm and that RuBisCO, but not PRK, also occurs in the carboxysomes of C. fritschii.
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