Abstract
CD8+ T cells are essential for host defense to intracellular bacterial pathogens such as Mycobacterium tuberculosis (Mtb), Salmonella species, and Listeria monocytogenes, yet the repertoire and dominance pattern of human CD8 antigens for these pathogens remains poorly characterized. Tuberculosis (TB), the disease caused by Mtb infection, remains one of the leading causes of infectious morbidity and mortality worldwide and is the most frequent opportunistic infection in individuals with HIV/AIDS. Therefore, we undertook this study to define immunodominant CD8 Mtb antigens. First, using IFN-γ ELISPOT and synthetic peptide arrays as a source of antigen, we measured ex vivo frequencies of CD8+ T cells recognizing known immunodominant CD4+ T cell antigens in persons with latent tuberculosis infection. In addition, limiting dilution was used to generate panels of Mtb-specific T cell clones. Using the peptide arrays, we identified the antigenic specificity of the majority of T cell clones, defining several new epitopes. In all cases, peptide representing the minimal epitope bound to the major histocompatibility complex (MHC)-restricting allele with high affinity, and in all but one case the restricting allele was an HLA-B allele. Furthermore, individuals from whom the T cell clone was isolated harbored high ex vivo frequency CD8+ T cell responses specific for the epitope, and in individuals tested, the epitope represented the single immunodominant response within the CD8 antigen. We conclude that Mtb-specific CD8+ T cells are found in high frequency in infected individuals and are restricted predominantly by HLA-B alleles, and that synthetic peptide arrays can be used to define epitope specificities without prior bias as to MHC binding affinity. These findings provide an improved understanding of immunodominance in humans and may contribute to a development of an effective TB vaccine and improved immunodiagnostics.
Highlights
Infection with Mycobacterium tuberculosis (Mtb) remains an important cause of infectious disease, morbidity, and mortality worldwide [1] and has emerged as a major opportunistic infection in individuals with HIV/AIDS [2]
CD8þ T cells are essential for host defense to intracellular bacterial pathogens such as Mycobacterium tuberculosis (Mtb), Salmonella species, and Listeria monocytogenes, yet little is known about the antigens recognized by human CD8þ T cells in response to tuberculosis (TB)
We measured the frequencies of CD8þ T cells recognizing Mtb antigens known to be recognized by CD4þ T cell antigens in persons infected with Mtb
Summary
Infection with Mycobacterium tuberculosis (Mtb) remains an important cause of infectious disease, morbidity, and mortality worldwide [1] and has emerged as a major opportunistic infection in individuals with HIV/AIDS [2]. B2microglobulin–deficient mice, deficient in expression of major histocompatibility complex (MHC) class I, are more susceptible to Mtb [9] and to large doses of Bacille Calmette Guerin [10] infection than their wild-type littermates. This finding has been corroborated in CD8-deficient mice [11] and other mice deficient in class I processing and presentation [11,12,13]. Studies of mice and humans support an important role for CD8þ T cells in TB immunity
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