Abstract
Mulberroside A is a major active compound from root barks of Morus alba L. (Moraceae). It is widely employed as an active ingredient in cosmetic products due to its anti-tyrosinase and anti-oxidant activities [1]. In order to verify the present of mulberroside A in large number of samples, a rapid and simple assay system is required to apply as small quantities utilization method. Previously, we reported enzyme-linked immunosorbent assay (ELISA) using highly specific mulberroside A polyclonal antibody for determination of mulberroside A in plant samples [2]. In this study, an immunochromatographic strip test was developed by using anti-mulberroside A polyclonal antibody. The qualitative assay was based on competitive immunoassay in which the detector reagent consisted of anti-mulberroside A polyclonal antibody colored with colloidal gold particles. The capture reagent was mulberroside A ovalbumin conjugate immobilized on the test strip membrane. The sample containing mulberroside A and the detector reagent were incubated together with immobilized capture reagent on nitrocellulose membrane. The detection limit for the strip test was 2 µg/mL. The developed immunochromatographic strip test was applied to determine mulberroside A in plants, medical preparations and cosmetic samples.
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