Abstract
Yeast Saccharomyces cerevisiae has been immobilized by adhesion to plate supports of glass or polycarbonate after a suitable pretreatment, and sedimentation on the support, followed by washing the non-adhering cells. The pretreatment consists of starving the cells in pure water. Its efficiency is related to both a release of substances, decreasing the electrostatic repulsion between the particles involved, and a modification of the cell wall itself. A single layer of immobilized cells is obtained and a large fraction of the cells keep the capacity to reduce methylene blue and to multiply. The influence of various experimental factors (pretreatment, harvesting time, time of contact with the support) is discussed.
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