Abstract
The permeability of polyimide–polyacrylic acid (PI-PAAc) pH-responsive membrane fabricated using a 248 nm KrF laser was investigated. These membranes were further immobilized with glucose oxidase enzyme, which led to the successful development of a glucose-responsive membrane. The base PI membranes were developed using a simple photolithographic technique. Further grafting of PAAc inside the pores was carried out using the same laser wavelength. The effect of various solution parameters and laser parameters were studied and discussed in detail in our previous work. A variety of grafting yields were obtained by changing laser exposure time. Glucose Oxidase (GOD) enzyme was then immobilized on the membrane using a carbodiimide-based amidation method. The polyacrylic acid grafted inside the pores shows pH-responsive gating. The immobilized GOD molecules show glucose sensitivity and convert the glucose into gluconic acid. The experiment results show that these membranes can detect the amount of glucose and release the corresponding solute amount.
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