Abstract
Effects of ion implantation into polystyrene (PS), silicone rubber (SR) and poly-l-lactic acid (PLA) have been investigated for immobilization of extracellular matrix. Carbon negative ions were implanted into PS and SR sheets at various energies between 5–30keV and various doses between 1.0×1014–1.0×1016 ions/cm2. Contact angles of pure water on C-implanted surfaces of PS and SR were decreased as increase in ion energy and in dose due to formation of functional groups such as OH and C–O. Selective attachment of nerve cells was observed on C-implanted them at 10keV and 3×1015 ions/cm2 after in vitro cell culture of nerve cells of PC-12h. Neurite outgrowth also extended over the implanted area. After dipping in a serum medium and in a fibronectin solution for 2h, the detection of N 1s electrons by X-ray induced photoelectron spectroscopic (XPS) revealed a significant distinction of protein adhesion on the implanted area. Thus, immobilization of proteins on surface is used for considering the selective cell-attachment. For PLA, the selective attachment of cells and protein depended on the implantation conditions.
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