Abstract
This work was designed to study the free and immobilized partial purified lovastatin in various applications. The results of HMG-CoA reductase inhibition showed enzyme inhibition at 10 mM of standard and partial purified lovastatin with specific activity 0.056 and 0.062 U/mg protein respectively, compared with specific activity 0.277 U/mg protein without inhibitor. The results of the thermal stability and storage time on lovastatin for inhibition of HMG-CoA reductase demonstrated that the standard and partial purified lovastatin were stabled in temperatures between 20-40 ᵒC, then the stability begun to decrease at 45 ᵒC, while lovastatin was stable in storage time between 1- 8 hours, then the stability begun to decrease after ten hours at 40 ºC. The results of MIC for lovastatin were demonstrated that most tested concentration were showed antibacterial activity of free and immobilized partial purified lovastatin against Candida albicans, Escherichia coli, and Staphylococcus aureus with MIC values ranging from 15 to 75 µg/ml. Whereas the results of minimum bactericidal concentration (MBC) and minimum fungicidal concentration (MFC) showed that C. albicans, E. coli, and S. aureus had no growth with concentration ranging from 55 to 75, 55 to 75, and 30 to 75 µg/ml, respectively. As well as the results of the cytotoxic impact using MTT experiment indicated that partial purified lovastatin caused a reduction in cells viability (p ≤ 0.05) at a dose-dependent manner on MCF-7 cell lines, with a calculating IC50 of 138.1 µg/ml, compare with normal cell line (WRL 68 Cell Line) at IC50 of 198.7 µg/ml.
Highlights
Lovastatin is polyketide components, created or produce via certain fungi during their secondary metabolism
The experiment was performed on the tumor in human glioblastoma cells and decrease in the malignancy was showed by lovastatin through inactivation of RAS farsonylation (29)
Lovastatin production from this isolate were performed by using a medium mention above and the growth was elicited with 1 ml (1x106 cells/ml)/5gm media of S.cerevisiae after 48 hours of culture
Summary
Lovastatin is polyketide components, created or produce via certain fungi during their secondary metabolism. CoA reductase inhibition: A 100 μl of standard and partial purified lovastatin separately were incubated in water bath at different temperature degrees (20, 25, 30, 35, 40, 45, 50, 55, and 60) OC for 30 min, the test tubes containing the lovastatin were transferred directly to cold water. Effect of different storage time of partial purified lovastatin on HMG-CoA reductase inhibition: A 100 μl of standard and partial purified lovastatin separately were incubated in water bath at 40 OC for different time [2, 4, 6, 8, 10, 24, 48] hours. Five wells (5 mm diameter) were made into (two plates) the agar via cork-borer and 0.1 ml of different solutions were applied in each well, these solution including the 75 μg/ml of standard lovastatin, partial purified lovastatin, immobilized lovastatin, nano-silver, and distilled water as control. ATR-FTIR analysis of partial purified lovastatin from local isolate A.terreus A50 using solid state fermentation
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