Abstract
Male pediatric survivors of cancers and bone marrow transplantation often require adjuvant chemoradiation therapy that may be gonadotoxic. The optimal methods to preserve fertility in these prepubertal males are still under investigation. This manuscript presents an in vivo experiment which involved transplantation of immature testicular tissues (ITT) from transgenic donor, to wild-type recipient mice. Donors and recipients were age-mismatched (from 20-week-old donors to 3-week-old recipients, and vice versa) and the transplantation sites involved the abdomen, skin of the head, back muscle, and scrotum. The application of poly-l-lactic acid (PLLA) scaffold was also evaluated in age-matched donors and recipients (both 3-weeks-old). To quantitively evaluate the process of spermatogenesis after ITT transplantation and scaffold application, bioluminescence imaging (BLI) was employed. Our result showed that ITT from 3-week-old mice had the best potential for spermatogenesis, and the optimal transplantation site was in the scrotum. Spermatogenesis was observed in recipient mice up to 51 days after transplantation, and up to the 85th day if scaffold was used. The peak of spermatogenesis occurred between the 42nd and 55th days in the scaffold group. This animal model may serve as a framework for further studies in prepubertal male fertility preservation.
Highlights
The arrowhead shows the significant spermatogenesis with mature sperm marked in with mature in yellow circleininscaffold the seminiferous tubules inand scaffold group yellow circlesperm in themarked seminiferous tubules group (Figure the control (Figure and the control group staining showed group (4C)
extracellular matrix (ECM) and appear to have a higher density of cells and sperm in addition to higher counts of sperm. Histological studies mirror those of the bioluminescence imaging (BLI) images, suggesting that scaffolds promoted enhanced cellular growth and graft survival. Both the histology and IHC support the fact that significantly better spermatogenesis occurred in immature testicular tissues (ITT) with a poly-L-lactic acid (PLLA) scaffold (Figure 4)
Two-tailed t tests were used for statistical analyses with p < 0.05 considered statistically significant. This translational bioengineering study may echo the messages from a cutting-edge review article, entitled “Fertility preservation for prepubertal boys: lessons learned from the past and update on remaining challenges towards clinical translation” [49]
Summary
Sites mashed testis tissueofon electrospun PLLA mats, instantly into isograft intoRecipient wild type recipient. Tissue of donors (FVB/N-Tg) from 20- or 3-week-old grafted into the scrotum, head, abdomen cavity, or back muscles of 3- and 20-week-old recipients (wt) was tracked using BLI for 51 days. Long-Term In Vivo Tracking of ITT Spermatogenesis in Age-Matched Donors and Recipients with/without Scaffold Bioengineering. The graftedComparitesticular were transplanted into the post-orchiectomy scrotum of age-matched tissues with scaffold could increase the degree of sperm proliferation compared to those son with or without a PLLA scaffold showed that the grafted testicular tissues with scaffold without the scaffold, in particular, on the 5th, 7th, and 42nd days without after transplantation Recipients (3-weeks-old), of transplanted age matched donor testicular tissue, to adults in vivo. Image showing the typical pattern of the representative in situ ITT engraftment in an individual
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
