Abstract
Continuous dispersion of pesticide in the environment and its stability in soil results in environmental pollution that cause harmful effect to living organisms and human by entering in food chain and this hazardous problem need to eradicate by remediation. The present research was attempted to isolate and characterize imidacloprid (IMIDA) degrading Bacillus strains from the different areas of Multan region. The imidacloprid resistant strains were isolated by checking their imidacloprid degradation ability in minimal salt medium (MSM). Levels of imidacloprid in MSM medium were analyzed by thin layer chromatography (TLC) and Bacillus mediated degradation of imidacloprid was assessed by using fourier transform infrared spectroscopy (FTIR). Out of 38 imidacloprid resistant strains, from 6 different soil samples, A2 showed highest imidacloprid degrading ability. The Bacillus strain A2 showed maximum growth at 25°C and pH 7 (at 600 nm). Degradation of IMIDA was determined by Rf values for IMIDA containing control (0.72) and culture extracts (0.8, 0.54 and 0.3) in TLC analysis. FTIR spectrum of strain A2 showed the appearance of new peaks at 2253 cm-1 from 2500 to 2000 cm-1 peak area as compared to control. The shifts of the peak pattern as well as occurrence of few new peaks attribute to biodegradation of imidacloprid after bacterial treatment. This study has shown that soil microbial activity significantly affected the technical profile of imidacloprid insecticides after soil application.
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