Illustration of the Distribution of DNA Sequences and Their Frequencies Within the MEP2 Gene Across Ten Candida Species

Abstract

Candida species have an impact on human skin health and occasionally can lead to catastrophic conditions, such as skin cancer. The nitrogen supply in growth media regulates MEP2 gene expression. MEP2 protein may regulate the amount of ammonium accessible to cells by directly affecting ammonium assimilation. Reducing MEP2 expression removed its potential to drive filamentous growth.This study aimed to examine serial imaging of the MEP2 gene with chaos game representation (CGR) and frequency chaos game representation (FCGR). In addition, the effect of mutations in Candida albicans strains on MEP2 docking with lauramine oxide (LDAO) was investigated. The MEP2 gene was selected for 10 Candida species from the National Center for Biotechnology Information to compare DNA sequences using conventional and portray methods (CGR and FCGR). The molecular docking between MEP2 and LDAO was determined using the HDOCK server.CGR findings revealed that Candida margitis, Candida orthopsilosis, Candida dubliniensis, Candida theae, and C albicans had approximately 65% of the same characteristics. According to FCGR, there was a 75% similarity between C albicans, C theae, C dubliniensis, C orthopsilosis, and C margitis. In certain strains, but not all a mutation in the conserved region of the protein caused a change in the docking residue of LDAO with MEP2.The CGR and FCGR protocols are considered practical and reliable tools for identifying protein and DNA sequence similarities. Approximately 80% of the existing algorithms for determining multiple sequence alignments are similar to traditional methods. Targeted treatment will be possible as determining MEP2 mutations is crucial for using Candida as a nitrogen source.